Title: Standard Operating Procedure – Probabilistic Reversal Learning (PRL)
Author: MouseTRAP Team
Date: 01/15/2020
Version: 1
Abstract: The probabilistic reversal learning (PRL) test has been designed to measure sensitivity to valence (i.e., positive and negative) feedback information. Previous use in operant boxes and touchscreen chambers has shown that PRL is sensitive to serotonergic manipulations, suggesting this test may be used to study depression-relevant behaviour in rodents. The test is performed in mice with specially designed touchscreen-based automated chambers with two response locations (far left and far right windows) using food reinforcement. Mice are required to respond to one of the two locations in every trial of a session, where one side is designated as optimal (S+) and the other is designated as suboptimal (S-). For every response to the optimal stimulus, there is an 80% chance of reward delivery and a 20% chance of no reward delivery; for every response to the suboptimal stimulus, there is an 80% chance of no reward delivery and a 20% chance of reward delivery. After 5 consecutive responses to the optimal stimulus, the reward conditions switch (i.e., the stimulus that was S+ is now S- and vice versa). Therefore, PRL requires within-session reversals.
DOI: 10.58064/wrcd-np77
Link to Full SOP: mPRL-v1
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