Title: Standard Operating Procedure – Five Choice Serial Reaction Time Task (5-CSRTT)
Author: MouseTRAP Team
Date: 11/28/2023
Version: 2
Abstract:The 5-CSRT task has been designed to measure effects of drugs and other manipulations (e.g., genetic) on attentional performance (and stimulus control). The test is performed in a specially designed touchscreen-based automated chamber with multiple response locations (’five-windows”) using food reinforcers to maintain performance. The 5-CSRT task is useful for measuring effects of different manipulations on various aspects of attentional control, including sustained, selective and divided attention – and is relevant to the definition of neural systems of attention and has applications to human disorders such as attention deficit/hyperactivity disorder (ADHD) and Alzheimer’s disease.
DOI: 10.58064/rm7j-3354
Link to Full SOP: m5CSRT-v2
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Version Notes
[DATE]: Version [#]
- Adjusted the After reward pause from 5s to 10s
- Reasoning is explained at the end of the SOP, but was based on fibre photometry recordings that demonstrated mice needed more time to baseline
- Included an additional formula for more data we can analyze: Premature Responses
- Added another method to run the stimulus duration probe
- Clarified details about the Inter Trial Interval
- Specifically, we made it clearer that the ITI is variable, rather than a constant duration between each trial