Title: Standard Operating Procedure – Heterogenous Sequence Task (HST)
Author: MouseTRAP Team
Date: 10/28/2021
Version: 1
Abstract: The heterogenous long sequence task is used measure complex motor learning and sequential response/response chain learning. The task is performed in specially designed touchscreen-based automated chambers with five response locations using food reinforcers to maintain performance. The sequence task requires the subject make five nose-pokes to white square stimuli appearing in five locations sequentially from left to right.
DOI: 10.58064/6r2v-fs32
Link to Full SOP: HetSequence-v1
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Version Notes
[DATE]: Version [#]
- Adjusted the After reward pause from 5s to 10s
- Reasoning is explained at the end of the SOP, but was based on fibre photometry recordings that demonstrated mice needed more time to baseline
- Included an additional formula for more data we can analyze: Premature Responses
- Added another method to run the stimulus duration probe
- Clarified details about the Inter Trial Interval
- Specifically, we made it clearer that the ITI is variable, rather than a constant duration between each trial